Which statement correctly describes bisulfite sequencing and how it detects DNA methylation?

Which statement correctly describes bisulfite sequencing and how it detects DNA methylation?

• A. Bisulfite converts all cytosines to thymine, regardless of methylation.
• B. Bisulfite converts unmethylated cytosines to uracil, which is read as thymine, while methylated cytosines remain as cytosine; sequencing reveals methylation status at single-base resolution.
• C. Bisulfite sequencing detects methylation by measuring DNA length differences.
• D. Bisulfite sequencing cannot distinguish unmethylated cytosines.

Answer: (B)